1st, as opposed to the kit's EDTA-that contains elution buffer, 10 mM Tris, pH7-8 is Commonly used for the ultimate elution, significantly for samples that are to become sequenced with PacBio chemistry, given that even small quantities of EDTA can impede downstream procedures included in sequencing library planning. Nuclease-free water https://www.facebook.com/NewTechFind/posts/pfbid0FDwXLmxi2raDNPcEpRa9jeGJbqTJ9rKxkuvCV6x8ZRC8R3wYhPw7yVXLXMNTF8JFl